Highly accurate spectrometer with broad range, ultraviolet through visible light illumination for detailed spectroscopic techniques in chemical structure and concentration determination.
- 1x Semi-Microvolume Cuvettes (Qty. 10)
- 1x Cuvette Rack
- 1x USB-A to USB-B Cable
- 1x External AC Adapter, 24 V Power Supply
- 1x Foam Lined Carrying Case (ABS)
See the Buying Guide for this item's required, recommended, and additional accessories.
Product Summary
The SE-3607 is an easy-to-use, wide range UV-Vis spectrometer that delivers fast, accurate and reliable performance for routine analyses in chemistry and biochemistry teaching labs. With USB connectivity and cross-platform Spectrometry Software, the PASCO UV-Vis Spectrometer improves collaboration between lab members, enabling data collected on a computer or laptop to be analyzed on tablets, iPads, and Chromebooks*. Additional accessories, such as the UV-Vis Fiber Optic Kit, can be used to extend the spectrometer’s capabilities for the analysis of emission spectra, light sources, and the classification of lasers.
* Chromebooks are not compatible with the PASCO UV-Vis Spectrometer for data collection (analysis only).
Free Spectroscopy Software
Overview
- Spectral scans from 180 to 1050 nm
- Intuitive, software-based operation
- Built-in tools for routine analyses
- One-click light and dark calibrations
- Clear marking to indicate proper cuvette positioning
The PASCO UV-Vis Spectrometer is durably designed to deliver top performance in fast-paced teaching labs.
- Compact, space-saving design
- High-sensitivity CMOS detector for faster analysis
- Internal drain reduces the risk of damage from drips and spills
- Isolated optic bench guarantees consistent accuracy over time (±1 nm)
- USB connectivity supports use with laboratory and student devices
- Compatible with a wide range of square and round cuvettes of 1-cm pathlengths
With wide range detection across the Visible, UVA, UVB, and UVC regions, the PASCO UV-Vis Spectrometer provides an exceptional standalone solution for routine applications such as:
- Kinetic measurements
- Purified protein analysis
- Determination of equilibrium constant
- Nucleic acid purity testing
- Quantification of DNA and RNA
- Analysis of extracted or synthesized compounds
- Determination of nucleic acid concentrations
- Colorimetric assays for protein quantification (e.g., Bradford, BCA, Lowry)
- Spectrophotometric determination of chemical and biochemical compounds
The PASCO UV-Vis Spectrometer is fully integrated with our user-friendly Spectrometry Software.
This free software is compatible with most student devices, enabling lab groups to quickly share and review their data with ease. Also available as a free, fully functional app, PASCO's cross-platform Spectrometry Software improves the efficiency of analyses with features such as:
- Easy-to-use navigation
- One-click light and dark calibrations
- Automatic display and storage of sample data
- Onboard scan averaging and data smoothing
- Intuitive run renaming for improved data tracking
- Spectral graph correlates visible wavelengths with color
- Built-in Beer-Lambert law with linear fits for determining concentrations
- Print spectrum and results snapshots
- Export data as a .csv file or .png screenshot for further analysis in Excel, SPARKvue, or PASCO Capstone
PASCO’s free Spectrometry Software includes four configured displays:
Analyze Solution: Absorbance vs. Wavelength
A sample of synthesized acetylsalicylic acid dissolved in ethanol was analyzed using the Analyze Solution screen. The sample's absorption spectrum indicates that the sample absorbs strongly at 237 and 313 nm.
Concentration: Absorbance vs. Concentration (Beer's Law)
The concentration of a purified protein was determined using the Beer-Lambert law within the Concentration screen. After selecting a target wavelength in the Analyze Solution screen, five protein standards (BSA) with known concentrations were analyzed. A linear fit was applied to create a standard curve, and the concentration of the unknown protein was determined to be 0.215 mmol/L.
Time: Absorbance vs. Time (Kinetics)
The fading of Phenolphthalein in NaOH was measured over time using the Time screen. The absorbance of the wavelength associated with Phenolphthalein was measured over time for samples with varying concentrations of NaOH. The results for a sample containing 0.3M NaOH and Phenolphthalein are provided below.
Analyze Light: Intensity vs. Wavelength
The Quartz Fiber Optic Cable Set was used to analyze the intensity of various spectral sources across the UV-Visible spectrum. The spectrum for Helium is shown below using the Analyze Light screen.
Applications
- Determination of solution concentrations
- Measurement of reaction rates or rate of decay
- Colorimetric assays (e.g., BCA, Bradford, Lowry)
- Purity testing of synthesized compounds
- Determination of the equilibrium constant
- Determination of molar absorption coefficients
- Quality testing (e.g., fermentation mediums, food adulteration, QA levels)
What's Included
- 1x Semi-Microvolume Cuvettes (Qty. 10)
- 1x Cuvette Rack (EC-3590)
- 1x USB-A to USB-B Cable
- 1x External AC Adapter, 24 V Power Supply
- 1x Foam Lined Carrying Case (ABS)
Product Specifications
Dimensions | 19.5 × 24.5 × 7.0 cm |
Light Source | Deuterium (UV) Tungsten (Vis) |
Detector | 2048 CMOS linear (0.3nm reporting interval) |
Wavelength Range | 180 to 1050 nm |
Wavelength Accuracy | 1.0 nm |
Grating | 500 lines/mm |
Signal to Noise | 2000:1 |
Optical Resolution | 1.5 nm |
Photometric Accuracy | ±5% |
Photometric Range (Best Accuracy) | 0.1 – 1.0 (reports full range 0.0 to 3.0) |
Typical Scan Time (Depends on Integration) | 4 ms – 10 s |
Connectivity | USB only |
Power Consumption | 2.5 A startup 350 mA continuous |
Power Supply | 100 - 240 VAC to 24VDC @ 2.5A |
Warranty | 5 year limited on hardware, 1 year on lamps |
Data Collection Software
This product requires PASCO software for data collection and analysis. We recommend the following option(s).
Connectivity Options
This product can connect directly to your computer or device with the following technologies. No Interface required.
- Universal Serial Bus (USB)
Buying Guide
Recommended Accessories | P/N | Price |
---|---|---|
Disposable UV Semi-Micro Volume Cuvettes (Qty. 10) | SE-3610 | -- |
UV Quartz Cuvettes (Qty. 2) | SE-3611 | -- |
UV-Vis Fiber Optic Kit | SE-7182 | -- |
Product Guides & Articles
Spectrometers
A spectrometer is a device that allows scientists to dissect light into its constituent colors, akin to separating the vibrant hues of a rainbow. By precisely dispersing the light, spectrometers provide a unique window into the physical and chemical properties of objects.
Spectroscopy
Spectroscopy is the investigation and measurement of spectra produced by matter interacting with or emitting electromagnetic radiation. During a spectroscopy experiment, electromagnetic radiation of a specified wavelength range passes from a source through a sample containing compounds of interest, resulting in absorption or emission.
Experiment Library
Perform the following experiments and more with the UV-Vis Spectrometer.
Visit PASCO's Experiment Library to view more activities.
Analysis of Aspirin Tablets
Students use a UV-Vis spectrometer to determine the mass of acetylsalicylic acid (ASA) in a single aspirin tablet and compare the results to the ASA content stated on the aspirin bottle.
Plant Pigments
Students analyze spinach pigments and chloroplasts using paper chromatography, a colorimeter, and a spectrometer to understand how plants capture light for photosynthesis.
Investigating Photosynthesis with Algae Beads
Students will use a Wireless Spectrometer, algae beads, and bicarbonate indicator to investigate the photosynthetic activity of algae and the effect on freshwater pH.
Percent Copper in Brass (Spectrometer)
Students use a spectrometer to create a Beer's law plot of known copper solution concentrations from which the unknown percent of copper in a brass sample can be determined.
Enzyme Activity (Spectrometer)
Students use a spectrometer to investigate the catalyzed breakdown of hydrogen peroxide by peroxidase.
Empirical Formula
In this lab, students use a spectrometer and stoichiometric calculations to obtain the chemical formula of a compound.
Sunscreens: Conjugation and UV Protection
Students use a UV-Vis spectrometer to observe the absorbance of various suncreens in the UVA and UVB regions of the electromagnetic spectrum, and relate sunscreen performance with molecular orbital structure and degree of conjugation...
Light, Color, and Concentration
In this lab, students use a spectrometer to learn how to use visible light to determine the concentration of colored ion species in a solution.
Chemical Equilibrium
Students use the results of a spectrometer and a temperature sensor to control the direction of a reversible chemical reaction.
Hydrogen Emission and Absorption Spectra
Students use a spectrometer, fiber optics cable, a hydrogen gas spectrum lamp, and the sun to compare emission and absorption lines, study atomic structure, associate light energy and wavelength with electron transitions, and...
Kinetics of Crystal Violet Fading (Spectrometer)
Students determine the reaction order of crystal violet fading in the presence of sodium hydroxide.
Support Documents
Manuals | ||
---|---|---|
UV-Vis Product Guide | English - 768.02 KB | |
Knowledge Base | ||
Increasing signal with spectrometers when used with optical fiber | Sep 18th, 2023 | |
Use a PASCO spectrometer with an older Mac | Mar 27th, 2023 | |
Use of a PASCO spectrometer with an older Windows computer | Mar 27th, 2023 |